SRX2263684
GSM2357278: Ehmt1 61WT frontcortex k9me3; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Neural
Cell type
Frontal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
whole frontal cortex
age
P30
tissue
brain
strain
C57B1/6J
antibody
k9me3
condition
Ehmt1 wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chipping for H3k9me3 (Diagenode A1671-001P) was performed as in http://www.blueprint-epigenome.eu/UserFiles/file/Protocols/Histone_ChIP_July2014.pdf Library was synthetized with KAPA Hyper prep kit (KK8504) according to manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
48757222
Reads aligned (%)
92.7
Duplicates removed (%)
37.6
Number of peaks
772 (qval < 1E-05)

mm9

Number of total reads
48757222
Reads aligned (%)
91.9
Duplicates removed (%)
37.3
Number of peaks
985 (qval < 1E-05)

Base call quality data from DBCLS SRA