Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

Cerebellum

MeSH Description

The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Sample

source_name

whole cerebellum

age

P30

tissue

brain

strain

C57B1/6J

antibody

k4me3

condition

Ehmt1+/-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chipping for H3k4me3 (Diagenode A5051-001P) was performed as in http://www.blueprint-epigenome.eu/UserFiles/file/Protocols/Histone_ChIP_July2014.pdf Library was synthetized with KAPA Hyper prep kit (KK8504) according to manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

47067024

Reads aligned (%)

97.5

Duplicates removed (%)

23.2

Number of peaks

25497 (qval < 1E-05)

mm9

Number of total reads

47067024

Reads aligned (%)

97.4

Duplicates removed (%)

23.3

Number of peaks

25504 (qval < 1E-05)