Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me3

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
shCTL stable cells, H3K79me3 ChIP
cell line
293T
stable shrna
shCTL
chip antibody
H3K79me3 (Abcam, ab2621, lot GR188147-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA fragment was obtained from IP. 1ug DNA was used for the construction of sequencing libraries. The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
19688559
Reads aligned (%)
96.8
Duplicates removed (%)
5.3
Number of peaks
5433 (qval < 1E-05)

hg19

Number of total reads
19688559
Reads aligned (%)
96.3
Duplicates removed (%)
5.3
Number of peaks
5252 (qval < 1E-05)

Base call quality data from DBCLS SRA