Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Mixed embryo
NA
NA

Attributes by original data submitter

Sample

source_name
whole embryo
developmental stage
mixed stage (embryonic)
strain
Bristol N2
genotype/variation
wild type
chip antibody
IgG (Sigma-Aldrich, catalog# I8140)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in Rechtsteiner, A. et al., 2010. Lysates were clarified from sonicated chromatin and histone-DNA complexes were isolated with antibody. Libraries were prepared using the ChIP-seq DNA sample prep kit (Illumina) with a protocol adapted from Meyer and Kircher, 2010.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
11010878
Reads aligned (%)
85.8
Duplicates removed (%)
18.8
Number of peaks
451 (qval < 1E-05)

ce10

Number of total reads
11010878
Reads aligned (%)
85.8
Duplicates removed (%)
18.8
Number of peaks
451 (qval < 1E-05)

Base call quality data from DBCLS SRA