GSM2349432: ChIP-seq Control Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESCs_ChIP-seq Control Input
strain background
CAST/Ei x 129/Sv/Jae
Sex
female
cell type
undifferentiated embryonic stem cells
genotype/variation
stable expression of control vector, clone 2D
molecule subtype
Genomic DNA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1.1% formaldeyde for 20 min, then nuclei were isolated and lysed. Chromatin was sheared by sonication to a size of 100-500bp. Cleared lysates were incubated overnight with anti-HA tag antibody, with 10% saved as input, then captured with Dynabeads Protein G. After stringent washes, ChIP DNA was eluted from beads, and together with input chromatin was reverse-crosslinked on 650C and treated with proteinase K. DNA was purified by phenol/chloroform extraction and ethanol precipitation. ChIP-seq libraries were constructed using the NEBNext ChIP-Seq Library Prep Master Mix Set (NEB). Multiplexed library, Ilumina barcode#3, TTAGGC. Paired-end 50bp reads