The hollow, muscular organ that maintains the circulation of the blood.
Attributes by original data submitter
Sample
source_name
Heart
sample type
Primary tissue
background strain
mixed background of C57BL/6 and 129S1
tissue
Heart
age
E12.5
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated by homogenization in hypotonic buffer and then resuspended in ChIP buffer for sonication. Sonication conditions were titrated to achieve sufficient fragmentation (mean fragment size 500 bp) while avoiding degradation of p300 protein. ChIP-seq libraries were prepared with KAPA Hyper Prep Kit (Kapa Biosystems)