Antigen

Antigen Class

TFs and others

Antigen

Med1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

treatment medium

Serum LIF

chip antibody

anti-Med1, Bethyl Laboratories A300-793A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

14976537

Reads aligned (%)

91.7

Duplicates removed (%)

7.7

Number of peaks

189 (qval < 1E-05)

mm9

Number of total reads

14976537

Reads aligned (%)

91.6

Duplicates removed (%)

7.9

Number of peaks

182 (qval < 1E-05)