Antigen

Antigen Class

TFs and others

Antigen

Brd4

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

treatment medium

Serum LIF + JQ1

chip antibody

anti-Brd4, Bethyl Laboratories A301-985A50

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

31581586

Reads aligned (%)

91.1

Duplicates removed (%)

25.4

Number of peaks

829 (qval < 1E-05)

mm9

Number of total reads

31581586

Reads aligned (%)

91.0

Duplicates removed (%)

26.0

Number of peaks

826 (qval < 1E-05)