Antigen

Antigen Class

TFs and others

Antigen

Nr1h4

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL6

Sex

male

treatment

TCA+Metformin

antibody

sc-13063

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as described in Flajollet,S., Lefebvre,B., Rachez,C., and Lefebvre,P. 2006. Distinct Roles of the Steroid Receptor Coactivator 1 and of MED1 in Retinoid-induced Transcription and Cellular Differentiation. J. Biol. Chem. 281:20338-20348. Libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina (E6240 and E7335 from New England Biolabs) according to the manufacturer's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

26843503

Reads aligned (%)

83.0

Duplicates removed (%)

8.3

Number of peaks

3779 (qval < 1E-05)

mm9

Number of total reads

26843503

Reads aligned (%)

82.8

Duplicates removed (%)

8.4

Number of peaks

3749 (qval < 1E-05)