GSM2340039: ChIPSeq siRAM 3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa Cells
cell line
HeLa
replicate
Replicate 3
treatment
siRNA against RAM
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA extracted using Trizol. ChIP-Seq performed as in Varshney et al, Nat. Comms, 2015 CLIP libraries prepared as in Huppertz et al, Methods, 2014 with a modified 3' RNA linker (3'-UGGAAUUCUCGGGUGCCAAGG-5'). RNASeq libraries were prepared according to Illumina's TruSeq Stranded Total RNA with Ribo-Zero Gold for Human/Mouse/Rat (RS-122-2303). ChIP-Seq libraries prepared as in Wiechens et al, PLoS genetics, 2016.