Antigen

Antigen Class

TFs and others

Antigen

Nr1h4

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver from animals with ligated bile duct, 14 days after surgery

surgery

bile duct ligation

chip antibody

FXR (Santa Cruz Biotechnology sc-13063)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.

Sequencing Platform

instrument_model

NextSeq 500

rn6

Number of total reads

28548890

Reads aligned (%)

84.8

Duplicates removed (%)

16.8

Number of peaks

8126 (qval < 1E-05)