Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

HOXA9 IDH1 R132H

cell type

HOXA9 immortalized bone marrow

treatment

Vitamin C

vector genotype

IDH1 R132H

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/) Genomic DNA/RNA was extracted using the Qiagen AllPrep Mini Kit according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

80532464

Reads aligned (%)

83.9

Duplicates removed (%)

7.9

Number of peaks

3997 (qval < 1E-05)

mm9

Number of total reads

80532464

Reads aligned (%)

83.8

Duplicates removed (%)

7.9

Number of peaks

3918 (qval < 1E-05)