Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

HOXA9 IDH1 R132H

cell type

HOXA9 immortalized bone marrow

treatment

Vitamin C

vector genotype

IDH1 R132H

chip antibody

Anti-H3K4me3; Cell Signaling Technology: Catalogue# 9751S, lot #8

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/) Genomic DNA/RNA was extracted using the Qiagen AllPrep Mini Kit according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

23561532

Reads aligned (%)

96.7

Duplicates removed (%)

5.2

Number of peaks

43074 (qval < 1E-05)

mm9

Number of total reads

23561532

Reads aligned (%)

96.6

Duplicates removed (%)

5.2

Number of peaks

43044 (qval < 1E-05)