Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

HFD, liver

strain

C57BL/6J

chip antibody

H3K27ac (Abcam, catalog# #4729, lot# GR132150-1)

treatment

HFD

age

19 weeks

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries of H3K27ac-enriched DNA were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279). Purified RNA was subjected to either polydT-enriched cDNA synthesis before sequencing and sequenced on the Illumina platform according to the manufacturer’s instructions. ChIP-seq and libraries of H3K27ac-enriched DNA and DHS-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

15374146

Reads aligned (%)

97.6

Duplicates removed (%)

24.2

Number of peaks

26534 (qval < 1E-05)

mm9

Number of total reads

15374146

Reads aligned (%)

97.5

Duplicates removed (%)

24.3

Number of peaks

26549 (qval < 1E-05)