GSM2325146: BirA Pulldown 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
HT22[BirA]
stably transfected construct
pSV40-zeo-BirA-V5
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% paraformaldehyde and 25 mM dithiobis(succinimydl propionate) and lysed with sodium dodecyl sulfate (SDS) buffer. Chromatin was sheared with a Covaris sonicator to 200-1000 bp fragments and protein-DNA complexes were isolated with Libraries were constructed using standard Illumina protocols.