GSM2319005: DnaJ1 ChIP 1 from WCE 1; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
DnaJ-1
Cell type
Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage
Attributes by original data submitter
Sample
source_name
Drosophila S2 cells, WT, DnaJ1 ChIP
cell line
S2
genotype/variation
WT
chip antibody
DnaJ1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was conducted on 1 x 10^7 cell equivalents of chromatin from Drosophila S2 cells. For input controls, 1 x 10^6 cell equivalents of chromatin was used. Soluble chromatin was incubated with rabbit α-MLF (gift from Dr. Anne Plessis), rat α-MLF (this work), rabbit α-DnaJ-1 (this work). ChIP-seq libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) or KAPA High Throughput Library Preparation Kit, Roche.