Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Pre-B cells

NA

NA

Sample

source_name

stromal adherent IKDN pre-B cell progenitors derived from IKDN CD2 Cre mice

cell type

stromal adherent pre-B cell progenitors

genotype

IKDN CD2 Cre

passages

3 to 8

antibody

EMD Millipore/07-449

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed according to previously described protocols (Zhang et al., NI 2011) and detailed in Hu et al., G&D 2016 Immunoprecipitated (ChIP) DNA and Input DNA were used to prepare sequencing libraries according to a modified version of the Illumina/Solexa Genomic DNA protocol and is detailed in Hu et al., G&D 2016.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

35263596

Reads aligned (%)

97.5

Duplicates removed (%)

4.6

Number of peaks

4847 (qval < 1E-05)

mm9

Number of total reads

35263596

Reads aligned (%)

97.4

Duplicates removed (%)

4.6

Number of peaks

4822 (qval < 1E-05)