Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa cells

genotype/variation

Wild-type

cell line

HeLa

antibody

anti-H3K9me3, ab8898 (Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Formaldehye-crosslinked chromatin was sheared by sonication to an average size of ~300bp and then immunoprecipitated with anti-H3K9me3 antibody ab8898 (Abcam) NEBNext ChIP-seq Library Prep Kit (NEB)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

93964276

Reads aligned (%)

96.9

Duplicates removed (%)

12.7

Number of peaks

42061 (qval < 1E-05)

hg19

Number of total reads

93964276

Reads aligned (%)

94.7

Duplicates removed (%)

15.6

Number of peaks

44885 (qval < 1E-05)