Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Uterus

Cell type

Endometriosis

MeSH Description

An enlarged area of ENDOMETRIOSIS that resembles a tumor. It is usually found in the OVARY. When it is filled with old blood, it is known as a chocolate cyst.

Sample

source_name

Endometriosis

cell type

hTERT immortalized endometriosis cells

genotype/variation

ARID1A Knockdown

antibody

H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were fixed, lysed, and chromatin was sonicated. Histone-DNA complexes were isolated with antibody. Genomic DNA was isolated by standard phenol-chloroform extraction and ethanol precipitation Libraries were prepared according to Illumina's instructions and sequenced on either HiSeq-2000 or NextSeq 500 platforms.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

16332012

Reads aligned (%)

90.7

Duplicates removed (%)

6.0

Number of peaks

3783 (qval < 1E-05)

hg19

Number of total reads

16332012

Reads aligned (%)

89.8

Duplicates removed (%)

7.5

Number of peaks

3720 (qval < 1E-05)