GSM7548117: MLL ChIP-seq in NT siRNA-transfected SEM cells [SEM NT MLL]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KMT2A
Cell type
Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
Leukemia cell line (SEM)
cell line
Leukemia cell line (SEM)
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
Bethyl A300-086A
treatment
NT siRNA transfection for 48h
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7548117
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (TFs). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).