GSM7516748: MUTZ3 - MYB ChIP-seq - Untreated; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYB
Cell type
Cell type Class
Blood
Cell type
MUTZ-3
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
Bone marrow
tissue
Bone marrow
cell line
MUTZ3
cell type
acute myelomonocytic leukemia
genotype
EVI1-eGFP
treatment
Untreated
antibody
Millipore, catalog no. 05-175
protocol
Double-crosslink DSG
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7516748
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and pulled down with antibody according to one of three different protocols: the Upstate histone protocol, with a single formaldehyde crosslink, or with a double DSG crosslink (see methods) ChIP samples were processed according to the Illumina TruSeq ChIP Sample Preparation Protocol (Illumina) or Diagenode Library V3 preparation protocol (Diagenode) and either sequenced single-end (1 × 50 bp) on the HiSeq 2500 platform (Illumina) or paired-end (2 × 100 bp) on the Novaseq 6000 platform (Illumina)