GSM7501616: HEK293, WT, MondoA ChIP, Replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MLXIP
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293
cell line
HEK293
cell type
Human embryonic kidney
knockdown
WT
chip antibody
MondoA (Proteintech 13614-1-AP)
replicate
2
batch
2
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7501616
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 5 min. After quenching and cell lysis chromatin was fragmented using QSonica Q800R sonicator. Total amount of 5 ug of human chromatin and 100 ng of Drosophila chromatin was used for IP. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit with the adapter dilution 1:25 and 5 uL pre-mixed forward/reverse primer solution. The starting mass of DNA for the library preparation was 5 ng. Libraries were amplified in 11 PCR cycles. Double size selection of DNA fragments of a size 200-600 bp was performed using magnetic AMPure XP beads (Beckman Coulter) and validated using Bioanalyzer High Sensitivity Chip (Agilent).