Antigen

Antigen Class

TFs and others

Antigen

Leo1

Cell type

Cell type Class

Muscle

Cell type

Myotube

NA

NA

Sample

source_name

Myotubes shPhf5a

cell type

mouse myotubes

chip antibody

Leo1 antibody, serum purified

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Ten million cells were used for the ChIP and precipitated using 5 micrograms of respective antibodies. Original reference: Zhu B. et al. (2005) "The human PAF complex coordinates transcription with events downstream of RNA synthesis" Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

56713857

Reads aligned (%)

95.7

Duplicates removed (%)

34.9

Number of peaks

761 (qval < 1E-05)

mm9

Number of total reads

56713857

Reads aligned (%)

95.2

Duplicates removed (%)

34.7

Number of peaks

903 (qval < 1E-05)