GSM7439579: EndoC-βH1, HBSS, TFEB 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TFEB
Cell type
Cell type Class
Pancreas
Cell type
EndoC-BH1
NA
NA
Attributes by original data submitter
Sample
source_name
EndoC-betaH1
cell line
EndoC-betaH1
cell type
Human pancreatic beta
chip antibody
TFEB
treatment
HBSS supplemented with HEPES
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7439579
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After the immunoprecipitation step, DNA was purified using AMPure XP beads. Briefly, beads were incubated with the reaction mix and washed several times using 70% Ethanol. Then, immunoprecipitated DNA was recovered by incubating with 10 mM Tris, pH 8.5, 0.1 mM EDTA buffer and used for DNA library preparation Libraries were prepared from 10 ng of DNA using the NEBNext UltraTM II DNA Library Prep Kit for Illumina (New England Biolabs)