GSM2293478: 786-O P300 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EP300
Cell type
Cell type Class
Kidney
Cell type
Renal cell carcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
ccRCC cell line
transcription factor
P300
chip antibody
P300 (sc-585, Santa Cruz)
source
786-O (ATCC CRL1932)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For each transcription factor, ~3x107 cells were cross-linked with 1% formaldehyde for 10 min at room temperature, and stopped by adding glycine to a final concentration of 0.2M. Chromatin was extracted and sonicated to ~500bp (Vibra cell, SONICS). The total volume of immunoprecipitation was 1.5 ml and the amount of antibody used was 15 µg. The input DNA was precleared with protein G Dynabeads (LifeTechnologies) for 2 hr at 4°C and then incubated with antibodies conjugated protein G beads overnight at 4°C. The beads were washed 6 times with wash buffer at room temperature. Amplified DNA was used with NEBNext ChIP-Seq library prep reagent set (NEB)