Antigen

Antigen Class

TFs and others

Antigen

PHF8

Cell type

Cell type Class

Kidney

Cell type

786-O

Primary Tissue

Kidney

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

786-O

cell line

786-O

cell type

clear cell renal cell carcinoma

transfection

siRNA targeting c-MYC

chip antibody

PHF8 (Bethyl, A301-772A)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM7401890

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes and quenched with 125 mM glycine. The DNA libraries were amplified for 15 cycles and sequenced using Illumina NovaSeq 6000 with paired-end 150 as the sequencing mode

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

33849426

Reads aligned (%)

81.6

Duplicates removed (%)

12.2

Number of peaks

3858 (qval < 1E-05)

hg19

Number of total reads

33849426

Reads aligned (%)

80.8

Duplicates removed (%)

12.5

Number of peaks

3154 (qval < 1E-05)