Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Gonad

Cell type

T80

NA

NA

Sample

source_name

T80 shArid1A Myr-PI3K

cell line background

T80

cell type

human ovarian epithelial cell line

genotype/variation

shArid1A Myr-PI3K

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Total RNA were extracted from samples using Trizol reagent. NEBNext Ultra Directional RNA Library Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. For DNase-seq, the nuclei were digested with DNase I (Roche) at 60 U/ml final concentration at 37°C for 5 minutes. The reactions were stopped by adding stop buffer containing EDTA and proteinase K. DNA was purified by phenyl-chloroform extraction and ethanol precipitation. Small fragments around 150 bp were selected with SPRIselect (Beckman Coulter). RNA-seq libraries were prepared following the manual of NEBNext Ultra Directional RNA Library Prep Kit.For DNase-seq, DNA obtained after DNase treatment were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

32477208

Reads aligned (%)

96.2

Duplicates removed (%)

7.7

Number of peaks

24446 (qval < 1E-05)

hg19

Number of total reads

32477208

Reads aligned (%)

95.6

Duplicates removed (%)

8.8

Number of peaks

24250 (qval < 1E-05)