Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

Glioma tumorspheres

NA

NA

Sample

source_name

H3K4me3 TS667

cell type

glioma tumorsphere

genotype

wild-type

passage

N/A

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using the Magna ChIP™ G - Chromatin Immunoprecipitation Kit (Millipore) according to manufacturer's instructions. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

27509138

Reads aligned (%)

60.6

Duplicates removed (%)

1.4

Number of peaks

20278 (qval < 1E-05)

hg19

Number of total reads

27509138

Reads aligned (%)

59.7

Duplicates removed (%)

1.5

Number of peaks

20159 (qval < 1E-05)