Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Th17 cells

cell type

Differentiated CD4+ T cells from lymph nodes and spleen (Th17 conditions: 4 days anti-CD3 (2ug/ml), anti-CD28 (5ug/ml), IL-6 (20ug/ml), IL-1b (10ug/ml), TGF-b (1ug/ml), anti-IFN-g(10ug/ml) after performed a mouse IL-17 secretion assay (Miltenyi, 130-094-212)

protocol

In vitro

strain

C57BL/6

antibody

H3K4me2 (Millipore, 07-030)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

36943322

Reads aligned (%)

86.6

Duplicates removed (%)

38.1

Number of peaks

41808 (qval < 1E-05)

mm9

Number of total reads

36943322

Reads aligned (%)

86.5

Duplicates removed (%)

38.2

Number of peaks

41835 (qval < 1E-05)