Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

Th1 cells

cell type

Differentiated CD4+ T cells from lymph nodes and spleen (Th1 conditions: 7 days anti-CD3 (10ug/ml), anti-CD28 (2ug/ml), IL-12 (5ug/ml), anti-IL-4 (10ug/ml)

protocol

In vitro

strain

C57BL/6

antibody

H3K4me2 (Millipore, 07-030)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

36488912

Reads aligned (%)

98.0

Duplicates removed (%)

12.7

Number of peaks

38724 (qval < 1E-05)

mm9

Number of total reads

36488912

Reads aligned (%)

97.9

Duplicates removed (%)

12.8

Number of peaks

38697 (qval < 1E-05)