Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
Control
chip antibody
H3K4me3; Millipore, #04-745

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA were extracted using antibodies for EPOP, H3K4me3, Elongin B, RNA Polymerase II S5, USP7 or H2Bub. Libraries were constructed of 10-50 ng of DNA following Illumina's® protocol

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
23379420
Reads aligned (%)
95.8
Duplicates removed (%)
15.0
Number of peaks
18483 (qval < 1E-05)

mm9

Number of total reads
23379420
Reads aligned (%)
95.6
Duplicates removed (%)
15.1
Number of peaks
18553 (qval < 1E-05)

Base call quality data from DBCLS SRA