Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

embryonic fibroblast chromatin

strain

C57BL/6

passage

4

cell type

E13.5 MEFs

growth condition

arrested

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was isolated from passage 4 MEFs cross-linked with ethylene glycol bissuccinimidylsuccinate and formaldehyde, then sonicated to ≤ 400 bp. DNA fragments were immunoprecipitated using different antibodies, washed, then purified. DNA was prepared for sequencing according to the Illumina TruSeq protocol. Illumina TruSeq manufacturers protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

19535530

Reads aligned (%)

85.7

Duplicates removed (%)

73.3

Number of peaks

234 (qval < 1E-05)

mm9

Number of total reads

19535530

Reads aligned (%)

85.5

Duplicates removed (%)

73.6

Number of peaks

254 (qval < 1E-05)