Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA4

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
purified cardiomyocyte G296S mutants
cell type
iPS-derived cardiomyocytes
passages
20-30
differentiation stage
Differentiation D32
chip antibody
GATA4 (sc1237, lot:E0912)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked cell pellets were lyzed, nuclei extracted, sonicated, immunoprecipitated, washed, reverse cross-link and column purified. Barcoded ChIP-seq libraries were made from ChIP DNA using the NuGen Ovation Ultralow DR or Diagenode Microplex Kit, checked for adapter artifacts by Agilent Bioanalyzer using DNA high sensitivity reagents and chips, and quantified by qPCR using an Illumina Library Quantification kit (KAPA Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
8306688
Reads aligned (%)
100.0
Duplicates removed (%)
0.0
Number of peaks
951 (qval < 1E-05)

hg38

Number of total reads
26171953
Reads aligned (%)
100.0
Duplicates removed (%)
0.0
Number of peaks
3233 (qval < 1E-05)

Base call quality data from DBCLS SRA