GSM7299937: CDK9 ER D538G Clone1 DMSO rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CDK9
Cell type
Cell type Class
Uterus
Cell type
Ishikawa
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ishikawa ER-D538G Clone 1
cell line
Ishikawa ER-D538G Clone 1
cell type
endometrioid adenocarcinoma
genotype
ESR1 mutant
chip antibody
CDK9 (Abcam ab239364)
treatment
1 hr 0.1% DMSO
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7299937
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For FLAG ChIPs (flag tagged ESR1), fixation was performed by treating cells with 1% formaldehyde for 10 minutes at room temperature. For CDK9 ChIPs, a double fixation was performed by treating cells with 2 mM disuccinimidyl glutarate (Thermo Scientific) for 20 minutes at room temperature followed by fixation with 1% formaldehyde for 10 minutes at room temperature. For both fixation methods, the cross-linking reaction was stopped with the addition of glycine to a final concentration of 125 mM. Cells were then washed with cold PBS and harvested via cell scraping in Farnham lysis buffer supplemented with protease inhibitors. Chromatin immunoprecipitation was performed as previously described (Reddy et al. Genome Research 2009) with an Anti-FLAG (Sigma-Aldrich M2) antibody that recognizes a FLAG tag on ESR1 or an Anti-CDK9 (Abcam ab239364) that recognizes CDK9.