Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Breast
Cell type
MDA-MB-468
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-468 cells
cell line
MDA-MB-468
treatment
ethanol
chip-seq antibody
anti-STAT3 (Santa Cruz Biotechnology, sc-482)
cell type
MDA-MB-468 (Human adenocarcinoma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
34806705
Reads aligned (%)
76.4
Duplicates removed (%)
12.5
Number of peaks
11050 (qval < 1E-05)

hg38

Number of total reads
34806705
Reads aligned (%)
77.5
Duplicates removed (%)
11.7
Number of peaks
11075 (qval < 1E-05)

Base call quality data from DBCLS SRA