Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SOX2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSC
cell line
iPSC
cell type
pluripotent stem cell
genotype
wt
treatment
No treatment
chip antibody
SOX2 (cell signaling 23064S)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM7246651
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei (Covaris S220), and protein-DNA complexes were isolated with antibody-conjugated Dynabeads NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs Inc.)

Sequencing Platform

instrument_model
NextSeq 2000

hg38

Number of total reads
28209093
Reads aligned (%)
97.5
Duplicates removed (%)
20.0
Number of peaks
552 (qval < 1E-05)

hg19

Number of total reads
28209093
Reads aligned (%)
97.2
Duplicates removed (%)
20.1
Number of peaks
492 (qval < 1E-05)

Base call quality data from DBCLS SRA