Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Muscle

Cell type

LHCN-M2

Tissue

skeletal muscle myoblast

Lineage

mesoderm

Description

skeletal myoblasts derived from satellite cells from the pectoralis major muscle of a 41 year old caucasian heart transplant donor, immortalized with lox-hygro-hTERT ("LH"), and Cdk4-neo ("CN"), Zhu et al. (2007) in Aging Cell, vol. 6, pp 515-523, newly promoted to tier 2: not in 2011 analysis.

Sample

source_name

LHCN-M2

biomaterial_provider

Dr. Woodring Wright

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

DnaseSeq

datatype description

DNaseI HS Sequencing

cell

LHCN-M2

cell organism

human

cell description

skeletal myoblasts derived from satellite cells from the pectoralis major muscle of a 41 year old caucasian heart transplant donor, immortalized with lox-hygro-hTERT ("LH"), and Cdk4-neo ("CN"), Zhu et al. (2007) in Aging Cell, vol. 6, pp 515-523, newly promoted to tier 2: not in 2011 analysis.

cell lineage

mesoderm

cell sex

M

treatment

DIFF_4d

treatment description

Myocytes differentiated from myoblasts for 4 days. See specific cell protocol for treatment details. (Stam)

labexpid

DS20647

labversion

Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75

replicate

1

Sequenced DNA Library

library_name

GSM1024771: UW_DnaseSeq_LHCN-M2_DIFF_4dRep1

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

101998645

Reads aligned (%)

89.7

Duplicates removed (%)

63.1

Number of peaks

151657 (qval < 1E-05)

hg19

Number of total reads

101998645

Reads aligned (%)

89.3

Duplicates removed (%)

63.8

Number of peaks

151369 (qval < 1E-05)