GSM7235294: SEM PINO RES biol rep 2 KMT2A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KMT2A
Cell type
Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
cell line
tissue
cell line
cell line
Acute Lymphoblastic Leukemia cell line SEM
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
genotype
t(4;11)(q21;q23) translocation
treatment
None
chip antibody
KMT2A ( Bethyl labs A300-086-A lot. 6)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7235294
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Up to 20 million leukemic cells were crosslinked and lysed using the SimpleChIP kit (Cell Signaling Technology®, #9003) according to the manufacturer's recommendations and subsequently sonicated using a Bioruptor sonicator (Diagenode) to generate 150-300 bp fragment size. Next, immunoprecipitation and antibody-protein-DNA precipitation were performed according to the guidelines of the manufacturer. Antibodies against KMT2A (Bethyl labs; cat.nr. A300-086-A), AFF1 (Abcam; cat.nr. ab31812), H3K4Me3, H3K27Ac and H3K79Me2 (Diagenode; cat. nrs. pAB-003-050, C15410196 and C15410051) were used. ChIP-seq DNA libraries were generated using the NEB Next Ultra DNA library preparation kit for Illumina (New England Biolabs) according to the manufacturer's recommendations ChIP-seq DNA libraries were generated using the NEB Next Ultra DNA library preparation kit for Illumina (New England Biolabs, E7645) according to the manufacturer's recommendations