Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

diet

low fat/low sucrose

tissue

liver

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Liver tissue was homogenized via Tissue Lyser II and DNA was extracted via DNeasy Blood and Tissue Kit per manufacturer’s instructions DNA libraries were prepared as per the SureSelectXT Methyl-Seq Target Enrichment System Protocol (Agilent) with a 9 cycle PCR post bisulfite conversion and a 6 cycle indexing PCR. SureSelect Mouse All Exon V1 52 Mbp was used for capture.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

62132054

Reads aligned (%)

95.0

Coverage rate (×)

2.8

Number of hyper MRs

189231 (qval < 1E-05)

mm9

Number of total reads

62132054

Reads aligned (%)

95.1

Coverage rate (×)

2.8

Number of hyper MRs

188828 (qval < 1E-05)