Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

Adult_DKO_Hepatocyte

strain

C57BL

tissue

Hepatocyte

age

15wk

aav injection time

1d

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

DNA was isolated from mouse hepatocytes or from snap-frozen mouse tissues and incubated in lysis buffer (25 mM Tris-HCl (pH  8), 2 mM EDTA, 0.2% SDS, 200 mM NaCl) supplemented with 300 μg/ml proteinase K (Roche) followed by phenol:chloroform extraction and ethanol precipitation. RRBS libraries were prepared as described (Boyle et al., 2012) and run on Hiseq 2500 (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

988525944

Reads aligned (%)

82.6

Coverage rate (×)

15.9

Number of hyper MRs

213117 (qval < 1E-05)

mm9

Number of total reads

988525944

Reads aligned (%)

83.6

Coverage rate (×)

16.0

Number of hyper MRs

213182 (qval < 1E-05)