Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Drosophila S2 cells

cell line

S2

treatment

Spt5-depleted

chip antibody

Pol II (anti-Rpb3, Drosophila; H-224, Santa Cruz Biotechnology #SC-9001, mouse)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Pol II ChIP material was prepared as described previously (Muse et al., 2007). Immunoprecipitated material was purified using the Qiaquick PCR purification kit

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

31888245

Reads aligned (%)

64.8

Duplicates removed (%)

2.0

Number of peaks

8069 (qval < 1E-05)

dm3

Number of total reads

31888245

Reads aligned (%)

65.0

Duplicates removed (%)

1.9

Number of peaks

8252 (qval < 1E-05)