Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Lung
Cell type
WI-38
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
WI-38
biomaterial_provider
Dr. Carl Mann, SBIGeM
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
WI-38
cell organism
human
cell description
embryonic lung fibroblast cells, hTERT immortalized, includes Raf1 construct
cell karyotype
normal
cell lineage
endoderm
cell sex
F
antibody
CTCF
antibody antibodydescription
Rabbit polyclonal. Antibody Target: CTCF
antibody targetdescription
CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendorname
Millipore
antibody vendorid
07-729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001903
labexpid
DS18348
labversion
Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75
replicate
2

Sequenced DNA Library

library_name
GSM1022634: UW_ChipSeq_WI-38_CTCFRep2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
18210516
Reads aligned (%)
71.9
Duplicates removed (%)
15.8
Number of peaks
18009 (qval < 1E-05)

hg38

Number of total reads
18210516
Reads aligned (%)
73.3
Duplicates removed (%)
14.7
Number of peaks
17986 (qval < 1E-05)

Base call quality data from DBCLS SRA