Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Lung
Cell type
NHLF
Tissue
lung
Lineage
endoderm
Description
lung fibroblasts

Attributes by original data submitter

Sample

source_name
NHLF
biomaterial_provider
Lonza
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
NHLF
cell organism
human
cell description
lung fibroblasts
cell karyotype
normal
cell lineage
endoderm
cell sex
U
antibody
CTCF
antibody antibodydescription
Rabbit polyclonal. Antibody Target: CTCF
antibody targetdescription
CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendorname
Millipore
antibody vendorid
07-729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH002043
labexpid
DS16349
labversion
Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75
replicate
1

Sequenced DNA Library

library_name
GSM1022626: UW_ChipSeq_NHLF_CTCFRep1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
32717581
Reads aligned (%)
78.4
Duplicates removed (%)
13.0
Number of peaks
32383 (qval < 1E-05)

hg38

Number of total reads
32717581
Reads aligned (%)
80.5
Duplicates removed (%)
11.4
Number of peaks
32424 (qval < 1E-05)

Base call quality data from DBCLS SRA