Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K8ac

Cell type

Cell type Class
Breast
Cell type
SK-BR-3
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
SKBR3 cells, H4K8ac ChIP
cell line
SKBR3
cell type
Breast cancer cell line
treatment
Control
chip antibody
H4K8ac (Millipore, 07-328, lot 2075956)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
66534374
Reads aligned (%)
91.5
Duplicates removed (%)
9.2
Number of peaks
24862 (qval < 1E-05)

hg19

Number of total reads
66534374
Reads aligned (%)
91.2
Duplicates removed (%)
10.0
Number of peaks
24754 (qval < 1E-05)

Base call quality data from DBCLS SRA