Antigen

Antigen Class

Histone

Antigen

H2BK120ub1

Cell type

Cell type Class

Breast

Cell type

SK-BR-3

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

SKBR3 cells, H2BK120ub1 ChIP

cell line

SKBR3

cell type

Breast cancer cell line

treatment

Control

chip antibody

H2BK120ub1 (Millipore, 05-1312, lot 2128268)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

30946326

Reads aligned (%)

88.1

Duplicates removed (%)

3.0

Number of peaks

4291 (qval < 1E-05)

hg19

Number of total reads

30946326

Reads aligned (%)

87.7

Duplicates removed (%)

3.5

Number of peaks

4355 (qval < 1E-05)