Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K8ac

Cell type

Cell type Class
Breast
Cell type
MDA-MB-361
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MB361 cells, H4K8ac ChIP
cell line
MB361
cell type
Breast cancer cell line
treatment
Control
chip antibody
H4K8ac (Millipore, 07-328, lot 2075956)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
57484085
Reads aligned (%)
94.6
Duplicates removed (%)
7.5
Number of peaks
12795 (qval < 1E-05)

hg19

Number of total reads
57484085
Reads aligned (%)
94.2
Duplicates removed (%)
8.5
Number of peaks
12792 (qval < 1E-05)

Base call quality data from DBCLS SRA