Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Breast
Cell type
SK-BR-3
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
SKBR3 cells, H3K27me3 ChIP
cell line
SKBR3
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
84902588
Reads aligned (%)
97.2
Duplicates removed (%)
6.3
Number of peaks
8144 (qval < 1E-05)

hg19

Number of total reads
84902588
Reads aligned (%)
96.8
Duplicates removed (%)
7.0
Number of peaks
8035 (qval < 1E-05)

Base call quality data from DBCLS SRA