Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Breast
Cell type
UACC-812
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
UACC812 cells, H3K4me1 ChIP
cell line
UACC812
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K4me1 (Abcam, ab8895, GR193882-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
29383223
Reads aligned (%)
88.8
Duplicates removed (%)
3.8
Number of peaks
29769 (qval < 1E-05)

hg19

Number of total reads
29383223
Reads aligned (%)
88.5
Duplicates removed (%)
4.0
Number of peaks
29686 (qval < 1E-05)

Base call quality data from DBCLS SRA