Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Breast

Cell type

MDA-MB-361

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MB361 cells, H3K27me3 ChIP

cell line

MB361

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

72893545

Reads aligned (%)

98.8

Duplicates removed (%)

4.2

Number of peaks

2670 (qval < 1E-05)

hg19

Number of total reads

72893545

Reads aligned (%)

98.4

Duplicates removed (%)

4.8

Number of peaks

2677 (qval < 1E-05)