Antigen

Antigen Class

Histone

Antigen

H3K79me2

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

MCF10A cells, H3K79me2 ChIP

cell line

MCF10A

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K79me2 (Abcam, ab3594, lot GR201271-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

41719485

Reads aligned (%)

89.6

Duplicates removed (%)

5.8

Number of peaks

12762 (qval < 1E-05)

hg19

Number of total reads

41719485

Reads aligned (%)

89.5

Duplicates removed (%)

5.9

Number of peaks

12769 (qval < 1E-05)